FastQCFastQC Report
Di 31 Jan 2017
ES-0004b_S18_L001_R2_001_20161113.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameES-0004b_S18_L001_R2_001_20161113.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904842
Sequences flagged as poor quality0
Sequence length35-301
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAA5400.0101.96325295
TATACAA4650.085.51756295
CCCTTAA5250.075.74413295
CACACTA1901.19720986E-464.39785295
CCTCTAA1600.00489714757.354332295
CTTAGAA1800.007803456450.98163295
GAATCTA1900.00966225448.298386295
GGACCTA1900.00966225448.298386295
CTTATAA7900.046.46427295
CTCCTAA2757.430769E-444.493057295
CAACCTA3150.001449454138.843143295
CTCTTAA3404.648197E-535.98703295
ACACACA5153.1627806E-635.637638295
ACAACAA9608.894858E-1031.863516295
TACCCAA3850.003878835631.780756295
TCACACA4903.346038E-431.213242295
TAGGACA2153.9555307E-430.3401584
TTAGTTC1800.006082646528.9917093
TTAAACA4301.7751518E-828.454863295
GGGCCCA4400.007450388727.80816295
Produced by FastQC (version 0.11.5)